Construct: shRNA TRCN0000018014

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015015.1-959s1c1
DNA Barcode:
GCCCATCATCCTGAAGAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KDM4B (23030)
Gene Description:
lysine demethylase 4B
Transcript:
RefSeq NM_015015.1 (NON-CURRENT)
Match location:
Position 959 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018014)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATCATCCTGAAGAAGTA
Hairpin Sequence:
5'-CCGG-GCCCATCATCCTGAAGAAGTA-CTCGAG-TACTTCTTCAGGATGATGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATCATCCTGAAGAAGTACTCGAGTACTTCTTCAGGATGATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATCATCCTGAAGAAGTACTCGAGTACTTCTTCAGGATGATGGGC-3'

Other clones with same target sequence:

  1. TRCN0000329961