Construct: shRNA TRCN0000018056

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006991.2-2492s1c1
DNA Barcode:
CGCTCAAACCTTATAGCCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF197 (10168)
Gene Description:
zinc finger protein 197
Transcript:
RefSeq NM_006991.2 (NON-CURRENT)
Match location:
Position 2492 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018056)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTCAAACCTTATAGCCCAT
Hairpin Sequence:
5'-CCGG-CGCTCAAACCTTATAGCCCAT-CTCGAG-ATGGGCTATAAGGTTTGAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTCAAACCTTATAGCCCATCTCGAGATGGGCTATAAGGTTTGAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTCAAACCTTATAGCCCATCTCGAGATGGGCTATAAGGTTTGAGCG-3'

Other clones with same target sequence:

(none)