Construct: shRNA TRCN0000018087

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013435.1-310s1c1
DNA Barcode:
GTTTACCAAGGACGACGGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAX (30062)
Gene Description:
retina and anterior neural fold homeobox
Transcript:
RefSeq NM_013435.1 (NON-CURRENT)
Match location:
Position 310 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018087)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTACCAAGGACGACGGGAT
Hairpin Sequence:
5'-CCGG-GTTTACCAAGGACGACGGGAT-CTCGAG-ATCCCGTCGTCCTTGGTAAAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTACCAAGGACGACGGGATCTCGAGATCCCGTCGTCCTTGGTAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTACCAAGGACGACGGGATCTCGAGATCCCGTCGTCCTTGGTAAAC-3'

Other clones with same target sequence:

(none)