Construct: shRNA TRCN0000018128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030935.3-1454s1c1
DNA Barcode:
CCGGAGGAAAGCTGTAGACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TSC22D4 (81628)
Gene Description:
TSC22 domain family member 4
Transcript:
RefSeq NM_030935.3 (NON-CURRENT)
Match location:
Position 1454 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAGGAAAGCTGTAGACAT
Hairpin Sequence:
5'-CCGG-CCGGAGGAAAGCTGTAGACAT-CTCGAG-ATGTCTACAGCTTTCCTCCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAGGAAAGCTGTAGACATCTCGAGATGTCTACAGCTTTCCTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAGGAAAGCTGTAGACATCTCGAGATGTCTACAGCTTTCCTCCGG-3'

Other clones with same target sequence:

(none)