Construct: shRNA TRCN0000018133

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_168302.4-1331s1c1
DNA Barcode:
CGGGAACAAAGTCTCACAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZKSCAN1 (7586)
Gene Description:
zinc finger with KRAB and SCAN domains 1
Transcript:
RefSeq XM_168302.4 (NON-CURRENT)
Match location:
Position 1331 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018133)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAACAAAGTCTCACAGAT
Hairpin Sequence:
5'-CCGG-CGGGAACAAAGTCTCACAGAT-CTCGAG-ATCTGTGAGACTTTGTTCCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAACAAAGTCTCACAGATCTCGAGATCTGTGAGACTTTGTTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAACAAAGTCTCACAGATCTCGAGATCTGTGAGACTTTGTTCCCG-3'

Other clones with same target sequence:

(none)