Construct: shRNA TRCN0000018238

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015070.2-1183s1c1
DNA Barcode:
CCTCTCCTTATCCTTCACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZC3H13 (23091)
Gene Description:
zinc finger CCCH-type containing 13
Transcript:
RefSeq NM_015070.2 (NON-CURRENT)
Match location:
Position 1183 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018238)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCCTTATCCTTCACATT
Hairpin Sequence:
5'-CCGG-CCTCTCCTTATCCTTCACATT-CTCGAG-AATGTGAAGGATAAGGAGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCCTTATCCTTCACATTCTCGAGAATGTGAAGGATAAGGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCCTTATCCTTCACATTCTCGAGAATGTGAAGGATAAGGAGAGG-3'

Other clones with same target sequence:

(none)