Construct: shRNA TRCN0000018330

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007110.x-166s1c1
DNA Barcode:
CAGATATCCTCTCCTTGAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEP1 (7011)
Gene Description:
telomerase associated protein 1
Transcript:
RefSeq NM_007110.3 (NON-CURRENT)
Match location:
Position 166 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018330)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATATCCTCTCCTTGAAGA
Hairpin Sequence:
5'-CCGG-CAGATATCCTCTCCTTGAAGA-CTCGAG-TCTTCAAGGAGAGGATATCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATATCCTCTCCTTGAAGACTCGAGTCTTCAAGGAGAGGATATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATATCCTCTCCTTGAAGACTCGAGTCTTCAAGGAGAGGATATCTG-3'

Other clones with same target sequence:

(none)