Construct: shRNA TRCN0000018339

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005027.x-1664s1c1
DNA Barcode:
CAGATGAAGCGTACTGCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3R2 (5296)
Gene Description:
phosphoinositide-3-kinase regulatory subunit 2
Transcript:
RefSeq NM_005027.1 (NON-CURRENT)
Match location:
Position 1664 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018339)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATGAAGCGTACTGCAATT
Hairpin Sequence:
5'-CCGG-CAGATGAAGCGTACTGCAATT-CTCGAG-AATTGCAGTACGCTTCATCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATGAAGCGTACTGCAATTCTCGAGAATTGCAGTACGCTTCATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATGAAGCGTACTGCAATTCTCGAGAATTGCAGTACGCTTCATCTG-3'

Other clones with same target sequence:

(none)