Construct: shRNA TRCN0000018449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173870.1-1392s1c1
DNA Barcode:
CCCTCTTAAGAGCGACAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mgat4a (269181)
Gene Description:
mannoside acetylglucosaminyltransferase 4, isoenzyme A
Transcript:
RefSeq NM_173870.1 (NON-CURRENT)
Match location:
Position 1392 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCTTAAGAGCGACAGTTT
Hairpin Sequence:
5'-CCGG-CCCTCTTAAGAGCGACAGTTT-CTCGAG-AAACTGTCGCTCTTAAGAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCTTAAGAGCGACAGTTTCTCGAGAAACTGTCGCTCTTAAGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCTTAAGAGCGACAGTTTCTCGAGAAACTGTCGCTCTTAAGAGGG-3'

Other clones with same target sequence:

(none)