Construct: shRNA TRCN0000018465

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138677.1-1993s1c1
DNA Barcode:
GCCCTTAAAGAGCATCTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Edem1 (192193)
Gene Description:
ER degradation enhancer, mannosidase alpha-like 1
Transcript:
RefSeq NM_138677.1 (NON-CURRENT)
Match location:
Position 1993 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018465)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTAAAGAGCATCTACAT
Hairpin Sequence:
5'-CCGG-GCCCTTAAAGAGCATCTACAT-CTCGAG-ATGTAGATGCTCTTTAAGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTAAAGAGCATCTACATCTCGAGATGTAGATGCTCTTTAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTAAAGAGCATCTACATCTCGAGATGTAGATGCTCTTTAAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000292840