Construct: shRNA TRCN0000018496

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007380.1-683s1c1
DNA Barcode:
CGACATCTTCTGGTGGATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abi1 (11308)
Gene Description:
abl-interactor 1
Transcript:
RefSeq NM_007380.1 (NON-CURRENT)
Match location:
Position 683 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018496)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACATCTTCTGGTGGATATA
Hairpin Sequence:
5'-CCGG-CGACATCTTCTGGTGGATATA-CTCGAG-TATATCCACCAGAAGATGTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACATCTTCTGGTGGATATACTCGAGTATATCCACCAGAAGATGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACATCTTCTGGTGGATATACTCGAGTATATCCACCAGAAGATGTCG-3'

Other clones with same target sequence:

  1. TRCN0000321712