Construct: shRNA TRCN0000018532

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003884.3-2346s1c1
DNA Barcode:
GTTGGCTATATCAAGGATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KAT2B (8850)
Gene Description:
lysine acetyltransferase 2B
Transcript:
RefSeq NM_003884.3 (NON-CURRENT)
Match location:
Position 2346 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018532)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGGCTATATCAAGGATTAT
Hairpin Sequence:
5'-CCGG-GTTGGCTATATCAAGGATTAT-CTCGAG-ATAATCCTTGATATAGCCAAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGGCTATATCAAGGATTATCTCGAGATAATCCTTGATATAGCCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGGCTATATCAAGGATTATCTCGAGATAATCCTTGATATAGCCAAC-3'

Other clones with same target sequence:

(none)