Construct: shRNA TRCN0000018536

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007114.1-2539s1c1
DNA Barcode:
GCCCAGCTAGAATCAGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMF1 (7110)
Gene Description:
TATA element modulatory factor 1
Transcript:
RefSeq NM_007114.1 (NON-CURRENT)
Match location:
Position 2539 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018536)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGCTAGAATCAGAGAAA
Hairpin Sequence:
5'-CCGG-GCCCAGCTAGAATCAGAGAAA-CTCGAG-TTTCTCTGATTCTAGCTGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGCTAGAATCAGAGAAACTCGAGTTTCTCTGATTCTAGCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGCTAGAATCAGAGAAACTCGAGTTTCTCTGATTCTAGCTGGGC-3'

Other clones with same target sequence:

  1. TRCN0000342839