Construct: shRNA TRCN0000018752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145128.2-1850s1c1
DNA Barcode:
GCAGAGAATCAACGCTTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mgat5 (107895)
Gene Description:
mannoside acetylglucosaminyltransferase 5
Transcript:
RefSeq NM_145128.2 (NON-CURRENT)
Match location:
Position 1850 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGAATCAACGCTTTCAT
Hairpin Sequence:
5'-CCGG-GCAGAGAATCAACGCTTTCAT-CTCGAG-ATGAAAGCGTTGATTCTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGAATCAACGCTTTCATCTCGAGATGAAAGCGTTGATTCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGAATCAACGCTTTCATCTCGAGATGAAAGCGTTGATTCTCTGC-3'

Other clones with same target sequence:

(none)