Construct: shRNA TRCN0000018760

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207237.1-947s1c1
DNA Barcode:
CGTTATGCGATGGGCAAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Man1c1 (230815)
Gene Description:
mannosidase, alpha, class 1C, member 1
Transcript:
RefSeq NM_207237.1 (NON-CURRENT)
Match location:
Position 947 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018760)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTATGCGATGGGCAAGAAT
Hairpin Sequence:
5'-CCGG-CGTTATGCGATGGGCAAGAAT-CTCGAG-ATTCTTGCCCATCGCATAACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTATGCGATGGGCAAGAATCTCGAGATTCTTGCCCATCGCATAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTATGCGATGGGCAAGAATCTCGAGATTCTTGCCCATCGCATAACG-3'

Other clones with same target sequence:

(none)