Construct: shRNA TRCN0000018781

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019804.1-688s1c1
DNA Barcode:
GCTGGACTACGGCATCTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B4galt4 (56375)
Gene Description:
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 4
Transcript:
RefSeq NM_019804.1 (NON-CURRENT)
Match location:
Position 688 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018781)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGACTACGGCATCTATAT
Hairpin Sequence:
5'-CCGG-GCTGGACTACGGCATCTATAT-CTCGAG-ATATAGATGCCGTAGTCCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGACTACGGCATCTATATCTCGAGATATAGATGCCGTAGTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGACTACGGCATCTATATCTCGAGATATAGATGCCGTAGTCCAGC-3'

Other clones with same target sequence:

(none)