Construct: shRNA TRCN0000018785

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019835.1-258s1c1
DNA Barcode:
CATAGTGAACACCTACCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B4galt5 (56336)
Gene Description:
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5
Transcript:
RefSeq NM_019835.1 (NON-CURRENT)
Match location:
Position 258 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018785)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAGTGAACACCTACCTCTT
Hairpin Sequence:
5'-CCGG-CATAGTGAACACCTACCTCTT-CTCGAG-AAGAGGTAGGTGTTCACTATG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAGTGAACACCTACCTCTTCTCGAGAAGAGGTAGGTGTTCACTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAGTGAACACCTACCTCTTCTCGAGAAGAGGTAGGTGTTCACTATG-3'

Other clones with same target sequence:

(none)