Construct: shRNA TRCN0000018808

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080445.2-937s1c1
DNA Barcode:
GTTGCGCCTTTCCTATGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B3galt6 (117592)
Gene Description:
UDP-Gal:betaGal beta 1,3-galactosyltransferase, polypeptide 6
Transcript:
RefSeq NM_080445.2 (NON-CURRENT)
Match location:
Position 937 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018808)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGCGCCTTTCCTATGTCTA
Hairpin Sequence:
5'-CCGG-GTTGCGCCTTTCCTATGTCTA-CTCGAG-TAGACATAGGAAAGGCGCAAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGCGCCTTTCCTATGTCTACTCGAGTAGACATAGGAAAGGCGCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGCGCCTTTCCTATGTCTACTCGAGTAGACATAGGAAAGGCGCAAC-3'

Other clones with same target sequence:

(none)