Construct: shRNA TRCN0000018820

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145933.2-1459s1c1
DNA Barcode:
CCAGACTACAACTTCTTCGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
St6gal1 (20440)
Gene Description:
beta galactoside alpha 2,6 sialyltransferase 1
Transcript:
RefSeq NM_145933.2 (NON-CURRENT)
Match location:
Position 1459 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018820)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGACTACAACTTCTTCGAA
Hairpin Sequence:
5'-CCGG-CCAGACTACAACTTCTTCGAA-CTCGAG-TTCGAAGAAGTTGTAGTCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGACTACAACTTCTTCGAACTCGAGTTCGAAGAAGTTGTAGTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGACTACAACTTCTTCGAACTCGAGTTCGAAGAAGTTGTAGTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000307367