Construct: shRNA TRCN0000018827

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009178.2-764s1c1
DNA Barcode:
CCACTGGATTGAGACCATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
St3gal4 (20443)
Gene Description:
ST3 beta-galactoside alpha-2,3-sialyltransferase 4
Transcript:
RefSeq NM_009178.2 (NON-CURRENT)
Match location:
Position 764 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018827)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTGGATTGAGACCATCTT
Hairpin Sequence:
5'-CCGG-CCACTGGATTGAGACCATCTT-CTCGAG-AAGATGGTCTCAATCCAGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTGGATTGAGACCATCTTCTCGAGAAGATGGTCTCAATCCAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTGGATTGAGACCATCTTCTCGAGAAGATGGTCTCAATCCAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000278752