Construct: shRNA TRCN0000018837

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009179.1-863s1c1
DNA Barcode:
CCAGATTTACAACCCAGCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
St3gal2 (20444)
Gene Description:
ST3 beta-galactoside alpha-2,3-sialyltransferase 2
Transcript:
RefSeq NM_009179.1 (NON-CURRENT)
Match location:
Position 863 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018837)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATTTACAACCCAGCCTT
Hairpin Sequence:
5'-CCGG-CCAGATTTACAACCCAGCCTT-CTCGAG-AAGGCTGGGTTGTAAATCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATTTACAACCCAGCCTTCTCGAGAAGGCTGGGTTGTAAATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATTTACAACCCAGCCTTCTCGAGAAGGCTGGGTTGTAAATCTGG-3'

Other clones with same target sequence:

  1. TRCN0000297210