Construct: shRNA TRCN0000018860

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002947.2-1229s1c1
DNA Barcode:
GCTAGCTCAATTCATCGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPA3 (6119)
Gene Description:
replication protein A3
Transcript:
RefSeq NM_002947.2 (NON-CURRENT)
Match location:
Position 1229 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018860)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGCTCAATTCATCGACAA
Hairpin Sequence:
5'-CCGG-GCTAGCTCAATTCATCGACAA-CTCGAG-TTGTCGATGAATTGAGCTAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGCTCAATTCATCGACAACTCGAGTTGTCGATGAATTGAGCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGCTCAATTCATCGACAACTCGAGTTGTCGATGAATTGAGCTAGC-3'

Other clones with same target sequence:

  1. TRCN0000281057