Construct: shRNA TRCN0000018865

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005657.1-6094s1c1
DNA Barcode:
GATACTTGGTCTTACTGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TP53BP1 (7158)
Gene Description:
tumor protein p53 binding protein 1
Transcript:
RefSeq NM_005657.1 (NON-CURRENT)
Match location:
Position 6094 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018865)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATACTTGGTCTTACTGGTTT
Hairpin Sequence:
5'-CCGG-GATACTTGGTCTTACTGGTTT-CTCGAG-AAACCAGTAAGACCAAGTATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATACTTGGTCTTACTGGTTTCTCGAGAAACCAGTAAGACCAAGTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATACTTGGTCTTACTGGTTTCTCGAGAAACCAGTAAGACCAAGTATC-3'

Other clones with same target sequence:

  1. TRCN0000229640