Construct: shRNA TRCN0000018956

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022482.3-1425s1c1
DNA Barcode:
CCACATGCTGATTTATCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GZF1 (64412)
Gene Description:
GDNF inducible zinc finger protein 1
Transcript:
RefSeq NM_022482.3 (NON-CURRENT)
Match location:
Position 1425 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000018956)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACATGCTGATTTATCATAA
Hairpin Sequence:
5'-CCGG-CCACATGCTGATTTATCATAA-CTCGAG-TTATGATAAATCAGCATGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACATGCTGATTTATCATAACTCGAGTTATGATAAATCAGCATGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACATGCTGATTTATCATAACTCGAGTTATGATAAATCAGCATGTGG-3'

Other clones with same target sequence:

(none)