Construct: shRNA TRCN0000019026

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007139.2-734s1c1
DNA Barcode:
CGGTCCTCAAATCTTACTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF92 (168374)
Gene Description:
zinc finger protein 92
Transcript:
RefSeq NM_007139.2 (NON-CURRENT)
Match location:
Position 734 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019026)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTCCTCAAATCTTACTAAA
Hairpin Sequence:
5'-CCGG-CGGTCCTCAAATCTTACTAAA-CTCGAG-TTTAGTAAGATTTGAGGACCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTCCTCAAATCTTACTAAACTCGAGTTTAGTAAGATTTGAGGACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTCCTCAAATCTTACTAAACTCGAGTTTAGTAAGATTTGAGGACCG-3'

Other clones with same target sequence:

(none)