Construct: shRNA TRCN0000019096

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001178.3-689s1c1
DNA Barcode:
CAACGCAATGTCCAGGAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARNTL (406)
Gene Description:
aryl hydrocarbon receptor nuclear translocator like
Transcript:
RefSeq NM_001178.3 (NON-CURRENT)
Match location:
Position 689 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019096)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACGCAATGTCCAGGAAATT
Hairpin Sequence:
5'-CCGG-CAACGCAATGTCCAGGAAATT-CTCGAG-AATTTCCTGGACATTGCGTTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACGCAATGTCCAGGAAATTCTCGAGAATTTCCTGGACATTGCGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACGCAATGTCCAGGAAATTCTCGAGAATTTCCTGGACATTGCGTTG-3'

Other clones with same target sequence:

(none)