Construct: shRNA TRCN0000019138

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002041.2-1073s1c1
DNA Barcode:
CCAGATGGACAACAAGTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABPB1 (2553)
Gene Description:
GA binding protein transcription factor subunit beta 1
Transcript:
RefSeq NM_002041.2 (NON-CURRENT)
Match location:
Position 1073 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019138)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATGGACAACAAGTATTA
Hairpin Sequence:
5'-CCGG-CCAGATGGACAACAAGTATTA-CTCGAG-TAATACTTGTTGTCCATCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATGGACAACAAGTATTACTCGAGTAATACTTGTTGTCCATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATGGACAACAAGTATTACTCGAGTAATACTTGTTGTCCATCTGG-3'

Other clones with same target sequence:

  1. TRCN0000245029