Construct: shRNA TRCN0000019145

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175709.1-293s1c1
DNA Barcode:
GTATAGGAAGAGAGGTCCGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBX7 (23492)
Gene Description:
chromobox 7
Transcript:
RefSeq NM_175709.1 (NON-CURRENT)
Match location:
Position 293 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019145)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATAGGAAGAGAGGTCCGAA
Hairpin Sequence:
5'-CCGG-GTATAGGAAGAGAGGTCCGAA-CTCGAG-TTCGGACCTCTCTTCCTATAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATAGGAAGAGAGGTCCGAACTCGAGTTCGGACCTCTCTTCCTATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATAGGAAGAGAGGTCCGAACTCGAGTTCGGACCTCTCTTCCTATAC-3'

Other clones with same target sequence:

(none)