Construct: shRNA TRCN0000019192

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000457.3-1145s1c1
DNA Barcode:
CGAGCAGATCCAGTTCATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HNF4A (3172)
Gene Description:
hepatocyte nuclear factor 4 alpha
Transcript:
RefSeq NM_000457.3 (NON-CURRENT)
Match location:
Position 1145 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019192)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCAGATCCAGTTCATCAA
Hairpin Sequence:
5'-CCGG-CGAGCAGATCCAGTTCATCAA-CTCGAG-TTGATGAACTGGATCTGCTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCAGATCCAGTTCATCAACTCGAGTTGATGAACTGGATCTGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCAGATCCAGTTCATCAACTCGAGTTGATGAACTGGATCTGCTCG-3'

Other clones with same target sequence:

(none)