Construct: shRNA TRCN0000019212

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006532.1-342s1c1
DNA Barcode:
CCTGGGCAGCATACAGGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELL (8178)
Gene Description:
elongation factor for RNA polymerase II
Transcript:
RefSeq NM_006532.1 (NON-CURRENT)
Match location:
Position 342 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019212)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGCAGCATACAGGACAA
Hairpin Sequence:
5'-CCGG-CCTGGGCAGCATACAGGACAA-CTCGAG-TTGTCCTGTATGCTGCCCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGCAGCATACAGGACAACTCGAGTTGTCCTGTATGCTGCCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGCAGCATACAGGACAACTCGAGTTGTCCTGTATGCTGCCCAGG-3'

Other clones with same target sequence:

(none)