Construct: shRNA TRCN0000019215

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001564.1-431s1c1
DNA Barcode:
CGGGCAAGACAAATGGAGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ING2 (3622)
Gene Description:
inhibitor of growth family member 2
Transcript:
RefSeq NM_001564.1 (NON-CURRENT)
Match location:
Position 431 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019215)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGCAAGACAAATGGAGTTA
Hairpin Sequence:
5'-CCGG-CGGGCAAGACAAATGGAGTTA-CTCGAG-TAACTCCATTTGTCTTGCCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGCAAGACAAATGGAGTTACTCGAGTAACTCCATTTGTCTTGCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGCAAGACAAATGGAGTTACTCGAGTAACTCCATTTGTCTTGCCCG-3'

Other clones with same target sequence:

(none)