Construct: shRNA TRCN0000019297

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002238.2-2211s1c1
DNA Barcode:
CGGAAGATCAGCGATGTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNH1 (3756)
Gene Description:
potassium voltage-gated channel subfamily H member 1
Transcript:
RefSeq NM_002238.2 (NON-CURRENT)
Match location:
Position 2211 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019297)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAGATCAGCGATGTGAAA
Hairpin Sequence:
5'-CCGG-CGGAAGATCAGCGATGTGAAA-CTCGAG-TTTCACATCGCTGATCTTCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAGATCAGCGATGTGAAACTCGAGTTTCACATCGCTGATCTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAGATCAGCGATGTGAAACTCGAGTTTCACATCGCTGATCTTCCG-3'

Other clones with same target sequence:

(none)