Construct: shRNA TRCN0000019299

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002051.1-1835s1c1
DNA Barcode:
GCCAAGAAGTTTAAGGAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GATA3 (2625)
Gene Description:
GATA binding protein 3
Transcript:
RefSeq NM_002051.1 (NON-CURRENT)
Match location:
Position 1835 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019299)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGAAGTTTAAGGAATAT
Hairpin Sequence:
5'-CCGG-GCCAAGAAGTTTAAGGAATAT-CTCGAG-ATATTCCTTAAACTTCTTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGAAGTTTAAGGAATATCTCGAGATATTCCTTAAACTTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGAAGTTTAAGGAATATCTCGAGATATTCCTTAAACTTCTTGGC-3'

Other clones with same target sequence:

(none)