Construct: shRNA TRCN0000019411

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003153.3-762s1c1
DNA Barcode:
GCTTGATAGAAACTCCTGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STAT6 (6778)
Gene Description:
signal transducer and activator of transcription 6
Transcript:
RefSeq NM_003153.3 (NON-CURRENT)
Match location:
Position 762 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019411)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGATAGAAACTCCTGCTA
Hairpin Sequence:
5'-CCGG-GCTTGATAGAAACTCCTGCTA-CTCGAG-TAGCAGGAGTTTCTATCAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGATAGAAACTCCTGCTACTCGAGTAGCAGGAGTTTCTATCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGATAGAAACTCCTGCTACTCGAGTAGCAGGAGTTTCTATCAAGC-3'

Other clones with same target sequence:

(none)