Construct: shRNA TRCN0000019467

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016169.2-643s1c1
DNA Barcode:
CTTTGGATAACAGTGAGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUFU (51684)
Gene Description:
SUFU negative regulator of hedgehog signaling
Transcript:
RefSeq NM_016169.2 (NON-CURRENT)
Match location:
Position 643 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019467)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGGATAACAGTGAGTCAA
Hairpin Sequence:
5'-CCGG-CTTTGGATAACAGTGAGTCAA-CTCGAG-TTGACTCACTGTTATCCAAAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGGATAACAGTGAGTCAACTCGAGTTGACTCACTGTTATCCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGGATAACAGTGAGTCAACTCGAGTTGACTCACTGTTATCCAAAG-3'

Other clones with same target sequence:

(none)