Construct: shRNA TRCN0000019469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015472.3-706s1c1
DNA Barcode:
GCGATGAATCAGCCTCTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WWTR1 (25937)
Gene Description:
WW domain containing transcription regulator 1
Transcript:
RefSeq NM_015472.3 (NON-CURRENT)
Match location:
Position 706 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATGAATCAGCCTCTGAAT
Hairpin Sequence:
5'-CCGG-GCGATGAATCAGCCTCTGAAT-CTCGAG-ATTCAGAGGCTGATTCATCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATGAATCAGCCTCTGAATCTCGAGATTCAGAGGCTGATTCATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATGAATCAGCCTCTGAATCTCGAGATTCAGAGGCTGATTCATCGC-3'

Other clones with same target sequence:

  1. TRCN0000319149