Construct: shRNA TRCN0000019470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015472.3-1327s1c1
DNA Barcode:
CCAGGAACAAACGTTGACTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WWTR1 (25937)
Gene Description:
WW domain containing transcription regulator 1
Transcript:
RefSeq NM_015472.3 (NON-CURRENT)
Match location:
Position 1327 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAACAAACGTTGACTTA
Hairpin Sequence:
5'-CCGG-CCAGGAACAAACGTTGACTTA-CTCGAG-TAAGTCAACGTTTGTTCCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAACAAACGTTGACTTACTCGAGTAAGTCAACGTTTGTTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAACAAACGTTGACTTACTCGAGTAAGTCAACGTTTGTTCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000319224