Construct: shRNA TRCN0000019544

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005967.2-1050s1c1
DNA Barcode:
CCGCAAATACAGCATCATCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAB2 (4665)
Gene Description:
NGFI-A binding protein 2
Transcript:
RefSeq NM_005967.2 (NON-CURRENT)
Match location:
Position 1050 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019544)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCAAATACAGCATCATCTA
Hairpin Sequence:
5'-CCGG-CCGCAAATACAGCATCATCTA-CTCGAG-TAGATGATGCTGTATTTGCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCAAATACAGCATCATCTACTCGAGTAGATGATGCTGTATTTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCAAATACAGCATCATCTACTCGAGTAGATGATGCTGTATTTGCGG-3'

Other clones with same target sequence:

(none)