Construct: shRNA TRCN0000019558

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014735.2-2086s1c1
DNA Barcode:
CCCACTAGAGAGCAAGAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JADE3 (9767)
Gene Description:
jade family PHD finger 3
Transcript:
RefSeq NM_014735.2 (NON-CURRENT)
Match location:
Position 2086 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019558)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTAGAGAGCAAGAATAA
Hairpin Sequence:
5'-CCGG-CCCACTAGAGAGCAAGAATAA-CTCGAG-TTATTCTTGCTCTCTAGTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTAGAGAGCAAGAATAACTCGAGTTATTCTTGCTCTCTAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTAGAGAGCAAGAATAACTCGAGTTATTCTTGCTCTCTAGTGGG-3'

Other clones with same target sequence:

(none)