Construct: shRNA TRCN0000019593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012429.1-721s1c1
DNA Barcode:
CCGAAACACTGAAGCGTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEC14L2 (23541)
Gene Description:
SEC14 like lipid binding 2
Transcript:
RefSeq NM_012429.1 (NON-CURRENT)
Match location:
Position 721 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAAACACTGAAGCGTCTTT
Hairpin Sequence:
5'-CCGG-CCGAAACACTGAAGCGTCTTT-CTCGAG-AAAGACGCTTCAGTGTTTCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAAACACTGAAGCGTCTTTCTCGAGAAAGACGCTTCAGTGTTTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAAACACTGAAGCGTCTTTCTCGAGAAAGACGCTTCAGTGTTTCGG-3'

Other clones with same target sequence:

(none)