Construct: shRNA TRCN0000019653

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014815.2-1980s1c1
DNA Barcode:
CTAGTGCAGATGAAGTGGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MED24 (9862)
Gene Description:
mediator complex subunit 24
Transcript:
RefSeq NM_014815.2 (NON-CURRENT)
Match location:
Position 1980 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019653)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGTGCAGATGAAGTGGCAT
Hairpin Sequence:
5'-CCGG-CTAGTGCAGATGAAGTGGCAT-CTCGAG-ATGCCACTTCATCTGCACTAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGTGCAGATGAAGTGGCATCTCGAGATGCCACTTCATCTGCACTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGTGCAGATGAAGTGGCATCTCGAGATGCCACTTCATCTGCACTAG-3'

Other clones with same target sequence:

(none)