Construct: shRNA TRCN0000019659

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003221.2-1658s1c1
DNA Barcode:
CGGTTCTTTCGAGTTTAGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TFAP2B (7021)
Gene Description:
transcription factor AP-2 beta
Transcript:
RefSeq NM_003221.2 (NON-CURRENT)
Match location:
Position 1658 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019659)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTCTTTCGAGTTTAGTAA
Hairpin Sequence:
5'-CCGG-CGGTTCTTTCGAGTTTAGTAA-CTCGAG-TTACTAAACTCGAAAGAACCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTCTTTCGAGTTTAGTAACTCGAGTTACTAAACTCGAAAGAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTCTTTCGAGTTTAGTAACTCGAGTTACTAAACTCGAAAGAACCG-3'

Other clones with same target sequence:

(none)