Construct: shRNA TRCN0000019874

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030763.1-252s1c1
DNA Barcode:
GCAGTTGCTGAAACCAAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HMGN5 (79366)
Gene Description:
high mobility group nucleosome binding domain 5
Transcript:
RefSeq NM_030763.1 (NON-CURRENT)
Match location:
Position 252 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019874)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTGCTGAAACCAAGCAA
Hairpin Sequence:
5'-CCGG-GCAGTTGCTGAAACCAAGCAA-CTCGAG-TTGCTTGGTTTCAGCAACTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTGCTGAAACCAAGCAACTCGAGTTGCTTGGTTTCAGCAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTGCTGAAACCAAGCAACTCGAGTTGCTTGGTTTCAGCAACTGC-3'

Other clones with same target sequence:

  1. TRCN0000342943