Construct: shRNA TRCN0000019921

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006286.1-1239s1c1
DNA Barcode:
CCCTGTTCGTTCAATGATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TFDP2 (7029)
Gene Description:
transcription factor Dp-2
Transcript:
RefSeq NM_006286.1 (NON-CURRENT)
Match location:
Position 1239 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019921)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTTCGTTCAATGATGAA
Hairpin Sequence:
5'-CCGG-CCCTGTTCGTTCAATGATGAA-CTCGAG-TTCATCATTGAACGAACAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTTCGTTCAATGATGAACTCGAGTTCATCATTGAACGAACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTTCGTTCAATGATGAACTCGAGTTCATCATTGAACGAACAGGG-3'

Other clones with same target sequence:

(none)