Construct: shRNA TRCN0000019925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013450.1-1025s1c1
DNA Barcode:
CCTCTGAAGAATCTAGTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BAZ2B (29994)
Gene Description:
bromodomain adjacent to zinc finger domain 2B
Transcript:
RefSeq NM_013450.1 (NON-CURRENT)
Match location:
Position 1025 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGAAGAATCTAGTTTAT
Hairpin Sequence:
5'-CCGG-CCTCTGAAGAATCTAGTTTAT-CTCGAG-ATAAACTAGATTCTTCAGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGAAGAATCTAGTTTATCTCGAGATAAACTAGATTCTTCAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGAAGAATCTAGTTTATCTCGAGATAAACTAGATTCTTCAGAGG-3'

Other clones with same target sequence:

(none)