Construct: shRNA TRCN0000019933

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006345.2-1356s1c1
DNA Barcode:
CCTTACTTCTATAACAGGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC30A9 (10463)
Gene Description:
solute carrier family 30 member 9
Transcript:
RefSeq NM_006345.2 (NON-CURRENT)
Match location:
Position 1356 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019933)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTACTTCTATAACAGGCAA
Hairpin Sequence:
5'-CCGG-CCTTACTTCTATAACAGGCAA-CTCGAG-TTGCCTGTTATAGAAGTAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTACTTCTATAACAGGCAACTCGAGTTGCCTGTTATAGAAGTAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTACTTCTATAACAGGCAACTCGAGTTGCCTGTTATAGAAGTAAGG-3'

Other clones with same target sequence:

(none)