Construct: shRNA TRCN0000019934

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014621.2-1145s1c1
DNA Barcode:
GCCCTCGGACTAGGTTAGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HOXD4 (3233)
Gene Description:
homeobox D4
Transcript:
RefSeq NM_014621.2 (NON-CURRENT)
Match location:
Position 1145 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000019934)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTCGGACTAGGTTAGCAT
Hairpin Sequence:
5'-CCGG-GCCCTCGGACTAGGTTAGCAT-CTCGAG-ATGCTAACCTAGTCCGAGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTCGGACTAGGTTAGCATCTCGAGATGCTAACCTAGTCCGAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTCGGACTAGGTTAGCATCTCGAGATGCTAACCTAGTCCGAGGGC-3'

Other clones with same target sequence:

(none)