Construct: shRNA TRCN0000020012

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005902.2-918s1c1
DNA Barcode:
CATCTCCTACTACGAGCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMAD3 (4088)
Gene Description:
SMAD family member 3
Transcript:
RefSeq NM_005902.2 (NON-CURRENT)
Match location:
Position 918 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020012)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCTCCTACTACGAGCTGAA
Hairpin Sequence:
5'-CCGG-CATCTCCTACTACGAGCTGAA-CTCGAG-TTCAGCTCGTAGTAGGAGATG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCTCCTACTACGAGCTGAACTCGAGTTCAGCTCGTAGTAGGAGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCTCCTACTACGAGCTGAACTCGAGTTCAGCTCGTAGTAGGAGATG-3'

Other clones with same target sequence:

(none)