Construct: shRNA TRCN0000020021

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006599.2-4698s1c1
DNA Barcode:
CCGAACTCAATTTCTCCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NFAT5 (10725)
Gene Description:
nuclear factor of activated T cells 5
Transcript:
RefSeq NM_006599.2 (NON-CURRENT)
Match location:
Position 4698 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020021)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAACTCAATTTCTCCACTT
Hairpin Sequence:
5'-CCGG-CCGAACTCAATTTCTCCACTT-CTCGAG-AAGTGGAGAAATTGAGTTCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAACTCAATTTCTCCACTTCTCGAGAAGTGGAGAAATTGAGTTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAACTCAATTTCTCCACTTCTCGAGAAGTGGAGAAATTGAGTTCGG-3'

Other clones with same target sequence:

(none)