Construct: shRNA TRCN0000020025

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016489.10-707s1c1
DNA Barcode:
GCCTTGAGGAATACAGAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NT5C3A (51251)
Gene Description:
5'-nucleotidase, cytosolic IIIA
Transcript:
RefSeq NM_016489.10 (NON-CURRENT)
Match location:
Position 707 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000020025)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGAGGAATACAGAATAT
Hairpin Sequence:
5'-CCGG-GCCTTGAGGAATACAGAATAT-CTCGAG-ATATTCTGTATTCCTCAAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGAGGAATACAGAATATCTCGAGATATTCTGTATTCCTCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGAGGAATACAGAATATCTCGAGATATTCTGTATTCCTCAAGGC-3'

Other clones with same target sequence:

(none)